Carryover effects minimized the positive effects of treated wastewater on anuran development.

Constructed wetlands (CWs) are a potential solution for wastewater treatment due to their capacity to support native species and provide tertiary wastewater treatment. However, CWs can expose wildlife communities to excess nutrients and harmful contaminants, affecting their development, morphology, and behavior. To examine how wastewater CWs may affect wildlife, we raised Southern leopard frogs, Lithobates sphenocephalus, in wastewater from conventional secondary lagoon and tertiary CW treatments for comparison with pondwater along with the presence and absence of a common plant invader to these systems - common duckweed (Lemna minor) - and monitored their juvenile development for potential carryover effects into the terrestrial environment. The tertiary CW treatment did not change demographic or morphological outcomes relative to conventional wastewater treatment in our study. Individuals emerging from both wastewater treatments demonstrated lower terrestrial survival rates than those emerging from pondwater throughout the experiment though experiment-wide survival rates were equivalent among treatments. Individuals from wastewater treatments transformed at larger sizes relative to those in pondwater, but this advantage was minimized in the terrestrial environment. Individuals that developed with duckweed had consistent but marginally better performance in both environments. Our results suggest a potential trade-off between short-term benefits of development in treated effluent and long-term consequences on overall fitness. Overall, we demonstrate that CWs for the purpose of wastewater treatment may not be suitable replicates for wildlife habitat and could have consequences for local population dynamics.

Interspecific interactions are conditional on temperature in an Appalachian stream salamander community.

Differences in the rates of responses to climate change have the potential to disrupt well-established ecological interactions among species. In semi-aquatic communities, competitive asymmetry based on body size currently maintains competitive exclusion and coexistence via interference competition. Elevated temperatures are predicted to have the strongest negative effects on large species and aquatic species. Our objectives were to evaluate the interaction between the effects of elevated temperatures and competitor identity on growth and habitat selection behavior of semi-aquatic salamanders in stream mesocosms. We observed interference competition between small and large species. Elevated temperatures had a negative effect on the larger species and a neutral effect on the smaller species. At elevated temperatures, the strength of interference competition declined, and the smaller species co-occupied the same aquatic cover objects as the larger species more frequently. Disruptions in competitive interactions in this community may affect habitat use patterns and decrease selection for character displacement among species. Determining how biotic interactions change along abiotic gradients is necessary to predict the future long-term stability of current communities.

Interactive effects of temperature and glyphosate on the behavior of blue ridge two-lined salamanders (Eurycea wilderae).

The objective of the present study was to evaluate the potential interactive effects of stream temperatures and environmentally relevant glyphosate-based herbicide concentrations on movement and antipredator behaviors of larval Eurycea wilderae (Blue Ridge two-lined salamander). Larval salamanders were exposed to 1 of 4 environmentally relevant glyphosate concentrations (0.00 µg acid equivalent [a.e.]/L, 0.73 µg a.e./L, 1.46 µg a.e./L, and 2.92 µg a.e./L) at either ambient (12 °C) or elevated (23 °C) water temperature. Behaviors observed included the exploration of a novel habitat, use of refuge, habitat selection relative to a potential predator, and burst movement distance. In the absence of glyphosate, temperature consistently affected movement and refuge-use behavior, with individuals moving longer distances more frequently and using refuge less at warm temperatures; however, when glyphosate was added, the authors observed inconsistent effects of temperature that may have resulted from differential toxicity at various temperatures. Larval salamanders made shorter, more frequent movements and demonstrated reduced burst distance at higher glyphosate concentrations. The authors also found that lower glyphosate concentrations sometimes had stronger effects than higher concentrations (i.e., nonmonotonic dose responses), suggesting that standard safety tests conducted only at higher glyphosate concentrations might overlook important sublethal effects on salamander behavior. These data demonstrate that sublethal effects of glyphosate-based herbicides on natural behaviors of amphibians can occur with short-term exposure to environmentally relevant concentrations. Environ Toxicol Chem 2016;35:2297-2303. © 2016 SETAC.

Effects of urbanization on occupancy of stream salamanders.

Urban development is the most common form of land conversion in the United States. Using a before-after control-impact study design, we investigated the effects of urbanization on larval and adult stages of southern two-lined salamanders (Eurycea cirrigera) and northern dusky salamanders (Desmognathus fuscus). Over 5 years, we estimated changes in occupancy and probabilities of colonization and survival in 13 stream catchments after urbanization and in 17 catchments that were not urbanized. We also examined effects of proportion of urbanized area in a catchment and distance of the salamander population to the nearest stream on probabilities of colonization and survival. Before urbanization, adult and larval stages of the two salamander species occupied nearly all surveyed streams, with occupancy estimates ranging from 1.0 to 0.78. Four years after urbanization mean occupancy of larval and adult two-lined salamanders had decreased from 0.87 and 0.78 to 0.57 and 0.39, respectively. Estimates of mean occupancy of larval northern dusky salamanders decreased from 1.0 to 0.57 in urban streams 4 years after urbanization; however, adult northern dusky salamander occupancy remained close to 1.0 in urban streams over 5 years. Occupancy estimates in control streams were similar for each species and stage over 5 years. Urbanization was associated with decreases in survival probabilities of adult and larval two-lined salamanders and decreases in colonization probabilities of larval dusky salamanders. Nevertheless, proportion of impervious surface and distance to nearest stream had little effect on probabilities of survival and colonization. Our results imply that in the evaluation of the effects of urbanization on species, such as amphibians, with complex life cycles, consideration of the effects of urbanization on both adult and larval stages is required.

Patterns of trombiculid mite (Hannemania dunni) parasitism among plethodontid salamanders in the western Piedmont of North Carolina.

Trombiculid mites are known to parasitize a variety of amphibian species, yet few comparisons of mite parasitism among amphibian species have been made. In this study, we investigated patterns of trombiculid mite parasitism among 3 plethodontid salamanders (Desmognathus fuscus, Eurycea cirrigera, and Plethodon cylindraceus) in the western Piedmont of North Carolina. All 3 salamander species were parasitized by a single species, Hannemania dunni. Desmognathus fuscus harbored mites more frequently (60.4% of individuals) than E. cirrigera (11.1%) or P. cylindraceus (14.6%). Desmognathus fuscus also had higher parasite loads than E. cirrigera or P. cylindraceus (P < 0.001). Mites on D. fuscus were found more frequently on the limbs than other body locations (P < 0.001). We found no correlation between salamander size and mite abundance (P = 0.689), but salamander collection sites influenced the abundance of mites on D. fuscus (P = 0.002). We found no effect of season on mite abundance in D. fuscus (P = 0.952). Salamander habitat preferences and edaphic or climatic differences among study sites may influence patterns of Hannemania sp. parasitism of salamanders.